The Transgenic Mouse Facility (TMF) is a core facility that provides services on a recharge basis for making, breeding, genotyping, importing, and preserving genetically-modified mice and embryonic stem cells. Advice is currently provided at no charge.
Got a question? Email the TMF - we're here to help!
"CRISPR" Cas9/gRNA genome editing now available !
Pictured below are founder mice generated by pronuclear injection of Cas9 mRNA and gRNA, targeting the Mitf locus (left) or hairless (hr) locus (right), into fertilized oocytes. Inquiries regarding gene targeting via Cas9 /gRNA should be directed to the TMF’s Managing Director, Jon Neumann.
Of interest to TMF and Cancer Center users
How can we assist you ?
- Finding and importing mice
- Need to import or export mice? Use Mustrac - an on-line tool developed at UCI
- Making mutant mice
- Designing a targeting vector
- What strain of mice did this ES cell line come from? (PDF)
The TMF is a Shared Resource funded in part by the Chao Family Comprehensive Cancer Center Support Grant (P30CA062203) from the National Cancer Institute, by Strategic Partnerships with various UCI Offices and Schools, and by established chargeback systems.