The Transgenic Mouse Facility (TMF) is a core facility that provides services on a recharge basis for making, breeding, genotyping, importing, and preserving genetically-modified mice and embryonic stem cells. Advice is currently provided at no charge.
Got a question? Email the TMF - we're here to help!
CRISPR/Cas9 genome editing is now available! Pictured below is a founder mouse generated in the TMF by pronuclear injection of Cas9 mRNA and sgRNA, targeting the Mitf locus, into fertilized C57BL/6N oocytes. Inquiries regarding gene targeting via CRISPR/Cas9 should be directed to the TMF’s Managing Director, Jon Neumann.
Of Interest to TMF Users
|CRISPR-mediated direct mutation of cancer genes in the mouse liver, Xue W, et al., Nature (2014) doi:10.1038/nature13589|
|Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype, Yin H, et al., Nature Biotechnology (2014) doi:10.1038/nbt.2884|
|'Humanized' Mouse Detects Deadly Drug Side Effects, J. Cohen, Science (2014) 344, p244|
|One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas9-Meditated Genome Engineering. Wang, et al., Cell (2013) 153, p910. DOI:10.1016/j.cell.2013.04.025|
|The APL Paradigm and the "Co-Clinical Trial" Project. Nardella, et al., Cancer Discovery (2011) 1, p108. DOI:10.1158/2159-8290.CD-11-0061|
|The IKMC Web Portal: a central point of entry to data and resources from the International Knockout Mouse Consortium. Ringwald, et al., Nucleic Acids Research (2011) 39, pD849.|
|BioMart as an integration solution for the International Knockout Mouse Consortium. Oakley, et al., Database (2011) bar028. doi:10.1093/database/bar028|
|A Resource of Vectors and ES Cells for Targeted Deletion of MicroRNAs in Mice. Prosser et al., Nature Biotechnology (2011) 29, p840.|
|A Rapid and Scalable System for Studying Gene Function in Mice using Conditional RNA Interference. Premsrirut et al., Cell (2011) 145, p145.|
How Can We Assist You?
- Finding and importing mice
- Need to import or export mice? Use Mustrac - an on-line tool developed at UCI
- Making mutant mice
- Designing a targeting vector
- What strain of mice did this ES cell line come from? (PDF)
The TMF is a Shared Resource funded in part by the Chao Family Comprehensive Cancer Center Support Grant (P30CA062203) from the National Cancer Institute, by Strategic Partnerships with various UCI Offices and Schools, and by established chargeback systems.