Transgenic Mouse Facility

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The Transgenic Mouse Facility (TMF) is a core facility that provides services on a recharge basis for making, breeding, genotyping, importing, and preserving genetically-modified mice and embryonic stem cells. Advice is currently provided at no charge.

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TMF News

CRISPR/Cas9 genome editing is now available! Pictured below is a founder mouse generated in the TMF by pronuclear injection of Cas9 mRNA and sgRNA, targeting the Mitf locus, into fertilized C57BL/6N oocytes.  Inquiries regarding gene targeting via CRISPR/Cas9 should be directed to the TMF’s Managing Director, Jon Neumann

  founder mouse with Mitf targeted by CRISPR/Cas9



Of Interest to TMF Users

Xue_et_al-pten_targeted_with_cas9.png CRISPR-mediated direct mutation of cancer genes in the mouse liver, Xue W, et al., Nature (2014) doi:10.1038/nature13589
Cas9FAHcorrection.jpg Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype, Yin H, et al., Nature Biotechnology (2014) doi:10.1038/nbt.2884
humanizedliver.jpg 'Humanized' Mouse Detects Deadly Drug Side Effects, J. Cohen, Science (2014) 344, p244
Jaenisch_CRISPR_pub050913.jpg One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas9-Meditated Genome Engineering. Wang, et al., Cell (2013) 153, p910. DOI:10.1016/j.cell.2013.04.025
cartoon of humans and mice The APL Paradigm and the "Co-Clinical Trial" Project. Nardella, et al., Cancer Discovery (2011) 1, p108. DOI:10.1158/2159-8290.CD-11-0061
Ringwald_et_al_NAR2011 The IKMC Web Portal: a central point of entry to data and resources from the International Knockout Mouse Consortium. Ringwald, et al., Nucleic Acids Research (2011) 39, pD849.
Oakley_et_al_Database2011 BioMart as an integration solution for the International Knockout Mouse Consortium. Oakley, et al., Database (2011) bar028. doi:10.1093/database/bar028
Prosser et al Nature Biotechnology 2011 A Resource of Vectors and ES Cells for Targeted Deletion of MicroRNAs in Mice.  Prosser et al., Nature Biotechnology (2011) 29, p840.
Premsrirut A Rapid and Scalable System for Studying Gene Function in Mice using Conditional RNA Interference. Premsrirut et al., Cell (2011) 145, p145.

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The TMF is a Shared Resource funded in part by the Chao Family Comprehensive Cancer Center Support Grant (P30CA062203) from the National Cancer Institute, by Strategic Partnerships with various UCI Offices and Schools, and by established chargeback systems.
Chao Family Comprehensive Cancer Center