Transgenic Mouse Facility


The Transgenic Mouse Facility (TMF) is a core facility that provides services on a recharge basis for making, breeding, genotyping, importing, and preserving genetically-modified mice and embryonic stem cells.

Got a question? Email the TMF - we're here to help!

"CRISPR" Cas9/gRNA genome editing now available !

Pictured below are founder mice generated by pronuclear injection of Cas9 mRNA and gRNA, targeting the Mitf locus (left) or hairless (hr) locus (right), into fertilized oocytes.  Inquiries regarding gene targeting via Cas9 /gRNA should be directed to the TMF’s Managing Director, Jon Neumann

  founder mouse with Mitf targeted by CRISPR/Cas9hairless pup at weaning

Of interest to TMF and Cancer Center users

Analysis of mammalian gene function through broad-based phenotypic screens across a consortium of mouse clinics. Hrabe de Angelis et al, (2015) Nature Genetics 47, p969.

CRISPR-mediated direct mutation of cancer genes in the mouse liver, Xue W, et al., Nature (2014) doi:10.1038/nature13589

Genome editing with Cas9 in adult mice corrects a disease mutation and phenotype, Yin H, et al., Nature Biotechnology (2014) doi:10.1038/nbt.2884

'Humanized' Mouse Detects Deadly Drug Side Effects, J. Cohen, Science (2014) 344, p244

The APL Paradigm and the "Co-Clinical Trial" Project. Nardella, et al., Cancer Discovery (2011) 1, p108. DOI:10.1158/2159-8290.CD-11-0061

How can we assist you ?

The TMF is a Shared Resource funded in part by the Chao Family Comprehensive Cancer Center Support Grant (P30CA062203) from the National Cancer Institute, by Strategic Partnerships with various UCI Offices and Schools, and by established chargeback systems.
Chao Family Comprehensive Cancer Center

News & Announcements

View More