The UC Irvine Transgenic Mouse Facility (TMF) is a core facility that provides services on a recharge basis for making, breeding, genotyping, importing, and preserving genetically-modified mice and embryonic stem cells. In addition to academic clients at UCI, we also service academic investigators at several other sister UC-campuses and numerous other universities throughout the USA as well as providing these services to commercial clients.
Got a question? Email the TMF - we're here to help!
"CRISPR" Cas9/gRNA genome editing services are available
In certain cases CRISPR Cas9/gRNA mediated gene editing can accelerate projects to modify the mouse genome. As of July 2016, the TMF has successfully completed 21 independent projects to modify the mouse genome via use of Cas9/gRNA. Most completed projects have involved introduction of point mutations, amino acid changes, loxP sites and exon deletions via Cas9/gRNA in combination with single-stranded oligodeoxynucleotide (ssODN) and homology dependent repair (HDR). This technology continues to evolve at a rapid pace and we routinely evaluate modifications of methods that may improve efficiency while using fewer animals.
Inquiries regarding gene targeting via Cas9 /gRNA should be directed to the TMF’s Managing Director, Jon Neumann.
Of interest to TMF and Cancer Center users
How can we assist you ?
- Finding and importing mice
- Need to import or export mice? Use Mustrac - an on-line tool developed at UCI
- Making mutant mice
- Designing a targeting vector
- What strain of mice did this ES cell line come from? (PDF)
The TMF is a Shared Resource funded in part by the Chao Family Comprehensive Cancer Center Support Grant (P30CA062203) from the National Cancer Institute, by Strategic Partnerships with various UCI Offices and Schools, and by established chargeback systems.