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Technical Advice

Welcome to the UC Irvine Transgenic Mouse Facility

The Transgenic Mouse Facility (TMF) is a core facility that provides services on a recharge basis for making, breeding, genotyping, importing, and preserving genetically-modified mice and embryonic stem cells. Advice is currently provided at no charge.

Got a question? Email the TMF - we're here to help!

TMF News

New Services:
- Sperm cryopreservation
- Targeted Transgenesis
New Capabilities:

Of Interest to TMF Users

The APL Paradigm and the "Co-Clinical Trial" Project. Nardella, et al., Cancer Discovery (2011) 1, p108. DOI:10.1158/2159-8290.CD-11-0061

The IKMC Web Portal: a central point of entry to data and resources from the International Knockout Mouse Consortium. Ringwald, et al., Nucleic Acids Research (2011) 39, pD849.

BioMart as an integration solution for the International Knockout Mouse Consortium. Oakley, et al., Database (2011) bar028. doi:10.1093/database/bar028

A Resource of Vectors and ES Cells for Targeted Deletion of MicroRNAs in Mice. Prosser et al., Nature Biotechnology (2011) 29, p840.

A Rapid and Scalable System for Studying Gene Function in Mice using Conditional RNA Interference. Premsrirut et al., Cell (2011) 145, p145.

How Can We Assist You?

Finding and importing mice
Need to import or export mice? Use Mustrac - an on-line tool developed at UCI
Making mutant mice
Designing a targeting vector
Breeding
Genotyping
What strain of mice did this ES cell line come from? (PDF)

The TMF is a Shared Resource funded in part by the Chao Family Comprehensive Cancer Center Support Grant (P30CA062203) from the National Cancer Institute, by Strategic Partnerships with various UCI Offices and Schools, and by established chargeback systems.

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